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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P2530-1KU | Recombinant Active Human COX1 (His & Twin-Strep-Tag) | 1KU | 1598.00元 |
| P2530-5KU | Recombinant Active Human COX1 (His & Twin-Strep-Tag) | 5KU | 5988.00元 |
| P2530-20KU | Recombinant Active Human COX1 (His & Twin-Strep-Tag) | 20KU | 18999.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 5742 | P23219 | HEK293 | 620aa | ~70kDa | C- His & Twin-Strep Tag |
| 蛋白信息(About this protein) | |
| 名称(Name) | Recombinant Active Human COX1 (His & Twin-Strep-Tag)，重组活性人源COX1 (His & Twin-Strep标签) |
| 别名(Synonyms) | Cyclooxygenase-1; Prostaglandin-endoperoxide synthase 1; Prostaglandin G/H Synthase 1; Prostaglandin H2 Synthase 1; COX-1; PGH synthase 1; PGHS1; PGHS-1; PTGHS; PHS 1 |
| 产品简介 (Background) |
Beyotime's recombinant human active form of human COX1 (rhCOX1) was expressed in HEK293 cells and purified, which contains the full length of COX1 (24-599aa) with a His tag (8X His) and a Twin-Strep tag (SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK) at the C-terminus. Cyclooxygenase is a bifunctional enzyme that first catalyzes the addition of two moles of molecular oxygen to arachidonic acid to form the hydroperoxide PGG2, then reduces the hydroperoxide to the alcohol, PGH2, by a peroxidase activity. Cyclooxygenase is found in two forms: COX1, which is constitutively expressed in most cells, is responsible for the production of prostaglandins that maintain homeostasis; and COX2, which is upregulated in inflammatory cells in response to an inflammatory stimulus (cytokines, LPS, etc.), is responsible for the production of prostaglandins at the site of inflammation [1]. |
| 产品用途 (Applications) |
Active human COX1 is useful in studying enzyme regulation, determining target substrates, screening COX1 inhibitors or as a positive control in COX1 activity assays. |
| 外观 (Physical appearance) |
Liquid |
| 浓度 (Concentration) |
Batch specific |
| 活性 (Biological activity) |
≥10U/μl |
| 活力单位 (Unit definition) |
One unit is defined as the amount of enzyme required to consume 1nmol of oxygen per 1 minute at 37°C in 100mM Tris pH8.0, 200μM arachidonic acid and 1.25μM hemin. |
| 纯度(Purity) | ≥90% by SDS-PAGE |
| 储存液 (Storage buffer) |
10mM HEPES (pH7.5), 100mM NaCl, 10% Glycerol |
本产品经SDS-PAGE电泳检测蛋白纯度和分子量请参考图1。
图1. 碧云天Recombinant Active Human COX1 (His & Twin-Strep-Tag) (P2530)的SDS-PAGE电泳检测效果图。本蛋白经BeyoGel™ Elite PAGE预制胶(Bis-Tris, 4-15%, 10孔) (P0856)电泳，Marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) (P0071/P0072)，并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际结果会因每批次蛋白浓度、实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。
本产品的酶活性(Biological activity)检测效果请参考图2。
图2. 碧云天Recombinant Active Human COX1 (His & Twin-Strep-Tag) (P2530)的酶活性检测效果图。X轴为COX1蛋白的质量(μg)，Y轴为对应单位时间内耗氧量(nmol/min)。实际结果因每批次蛋白浓度、实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。
本产品可用于COX1抑制剂的筛选。本产品用于检测NS-398抑制剂(S1771)的IC50约为83.72μM，用于检测Curcumin抑制剂(SC0299)的IC50约为1.56μM，检测效果请参考图3。
图3. 碧云天Recombinant Active Human COX1 (His & Twin-Strep-Tag) (P2530)用于抑制剂筛选的检测效果图。实际结果因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。
包装清单：| 产品编号 | 产品名称 | 包装 |
| P2530-1KU | Recombinant Active Human COX1 (His & Twin-Strep-Tag) (10U/μl) | 100μl |
| P2530-5KU | Recombinant Active Human COX1 (His & Twin-Strep-Tag) (10U/μl) | 500μl |
| P2530-20KU | Recombinant Active Human COX1 (His & Twin-Strep-Tag) (10U/μl) | 500μl×4 |
| — | 说明书 | 1份 |
-80ºC保存，一年有效。避免反复冻融。
注意事项：本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| P2530 | Recombinant Active Human COX1 (His & Twin-Strep-Tag) | 1/5/20KU |
| P2532 | Recombinant Active Human COX2 (His & Twin-Strep-Tag) | 1/5/20KU |
| S0165S | 环氧化酶-1 (COX-1)抑制剂筛选试剂盒 | 100次 |
| S0168 | 环氧化酶-2 (COX-2)抑制剂筛选试剂盒 | 100次 |
| S1771 | NS-398 (COX-2抑制剂) | 1mg |
| S1772 | NS-398 (COX-2抑制剂) | 5mg |
| SC0299-10mM | Curcumin (COX抑制剂) | 10mM×0.2ml |
| SC0299-5mg | Curcumin (COX抑制剂) | 5mg |
| SC0299-25mg | Curcumin (COX抑制剂) | 20mg |
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