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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P5089-1mg | Monomeric Streptavidin (单体链霉亲和素, powder) | 1mg | 812.00元 |
| P5089-5mg | Monomeric Streptavidin (单体链霉亲和素, powder) | 5mg | 3058.00元 |
| P5089-20mg | Monomeric Streptavidin (单体链霉亲和素, powder) | 20mg | 9178.00元 |
| P5089-100mg | Monomeric Streptavidin (单体链霉亲和素, powder) | 100mg | 32638.00元 |
碧云天研发生产的Monomeric Streptavidin (单体链霉亲和素, powder)是一种在大肠杆菌中重组表达纯化的蛋白，可用于生物素(Biotin)标记的抗体、核酸、蛋白、糖类、脂质等的检测，适用于活体细胞表面标记、细胞成像、体内近端生物素化、与标记物(如辣根过氧化物等)的连接、流式细胞检测、免疫组织化学、和抗体纯化等实验。
单体链霉亲和素分子量为15KDa，与天然重组链霉亲和素四聚体(55KDa)相比，两者结合生物素的能力相当，但与生物素标记物的结合效果更佳，总体比四聚体更能有效避免空间位置的阻碍效应，同时还可以避免四聚链霉亲和素引起的交联效应而导致的聚集或沉淀。单体链霉亲和素的大小和单价特性在如功能性细胞表面标记和原子力光谱等应用中提供了优于四聚链霉亲和素的优势：单体链霉亲和素不会与生物素标记的细胞、抗原、抗体、核酸、蛋白、多肽、凝集素等分子结合时因交联而形成聚集或沉淀；具有良好的均一性，实验的可重复性更好；体积更小，在空间上更容易接触目标，标记效果更好；最小化探测生物素化靶标的分布或动力学[1-4]。
本产品N端融合His标签，C端融合Flag标签，方便进行后续检测和纯化。
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Streptomyces avidinii | - | - | E. coli | 138aa | 15kDa | N-His, C-Flag |
| About this protein | |
| Name | Monomeric Streptavidin (Recombinant monoStreptavidin; mSA)；单体链霉亲和素 |
| Synonyms | N/A |
| Purity | >98% by SDS-PAGE |
| Biological Activity | >8U/mg |
| A282 | 2.8 (0.1% solution) |
| Formulation | lyophilized powder |
| Endotoxin | Less than 0.1EU/μg of monomeric streptavidin as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to precipitate the powder to the bottom. Reconstitute in ultrapure water or PBS to a concentration of 1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20ºC. Further dilutions should be made in appropriate buffered solutions. |
| Category | Streptavidin |
| Background | Monomeric Streptavidin is a mutant of Streptavidin, which is engineered by introducing mutations at the subunit interface and redesigning the binding pocket. The molecular mass of monomeric streptavidin is ~15kDa. Monomeric Streptavidin has only one binding site for biotin, but its engineering dissociation constant (KD) is like that of streptavidin [1-4]. |
| Amino Acid Sequence | MHHHHHHGSA EAGITGTWY NQRGSTFTVT AGADGNLTGQ YENRAQGWGC QNSPYTLTGR YNGTKLEWRV EWNNSTENCH SRTEWRGQY QGGAEARINT QWNLTYEGGS GPATTQGQDT FTKVKPSAAS GSDYKDDDDK |
| 产品编号 | 产品名称 | 包装 |
| P5089-1mg | Monomeric Streptavidin (powder) | 1mg |
| P5089-5mg | Monomeric Streptavidin (powder) | 5mg |
| P5089-20mg | Monomeric Streptavidin (powder) | 20mg |
| P5089-100mg | Monomeric Streptavidin (powder) | 100mg |
| — | 说明书 | 1份 |
-20ºC保存，至少两年有效。
注意事项：由于蛋白的每次冻融均会引起部分失活，首次使用时建议溶解并分装后-20ºC或更低温度冻存，以避免反复冻融。
本产品溶解后可能会有少量难溶物，离心去除后不影响蛋白活性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| P2151-200μl | BeyoMag™ Streptavidin Magnetic Beads (链霉亲和素磁珠) | 200μl |
| P2151-1ml | BeyoMag™ Streptavidin Magnetic Beads (链霉亲和素磁珠) | 1ml |
| P2151-5ml | BeyoMag™ Streptavidin Magnetic Beads (链霉亲和素磁珠) | 5ml |
| P5084-1mg | Recombinant Streptavidin | 1mg |
| P5084-5mg | Recombinant Streptavidin | 5mg |
| P5084-20mg | Recombinant Streptavidin | 20mg |
| P5084-100mg | Recombinant Streptavidin | 100mg |
| P5087-1mg | Monomeric Streptavidin (单体链霉亲和素) | 1mg |
| P5087-5mg | Monomeric Streptavidin (单体链霉亲和素) | 5mg |
| P5087-20mg | Monomeric Streptavidin (单体链霉亲和素) | 20mg |
| P5087-100mg | Monomeric Streptavidin (单体链霉亲和素) | 100mg |
| P5089-1mg | Monomeric Streptavidin (单体链霉亲和素, powder) | 1mg |
| P5089-5mg | Monomeric Streptavidin (单体链霉亲和素, powder) | 5mg |
| P5089-20mg | Monomeric Streptavidin (单体链霉亲和素, powder) | 20mg |
| P5089-100mg | Monomeric Streptavidin (单体链霉亲和素, powder) | 100mg |
| A0312 | 碱性磷酸酯酶标记Streptavidin | 0.2ml |
| A0303 | 辣根过氧化物酶标记Streptavidin | 0.2ml |
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